Regulatory

Part:BBa_K354003:Design

Designed by: Gregory Meyer   Group: iGEM10_WITS-South_Africa   (2010-10-20)
Revision as of 11:19, 20 October 2010 by Registry (Talk | contribs)

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PlcR Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

So as to create a library of promoters with differing transcriptional activities, randomised nucleotides were introduced into the -10 box - the binding site for the RNAP sigma-factor.


Source

The sequence is derived from the PlcR promoter in Bacillus thuringiensis and was synthesized via primer extension.

References