Regulatory

Part:BBa_K354003:Design

Designed by: Gregory Meyer   Group: iGEM10_WITS-South_Africa   (2010-10-20)

PlcR Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

So as to create a library of promoters with differing transcriptional activities, randomised nucleotides were introduced into the -10 box - the binding site for the RNAP sigma-factor.


Source

The sequence is derived from the PlcR promoter in Bacillus thuringiensis and was synthesized via primer extension.

References

[1] Lereclus D., Agaisse H., Gominet M., Salamitou S. & Sanchis V. (1996). Identification of a Bacillus thuringiensis Gene That Positively Regulates Transcription of the Phosphatidylinostitol-Specific Phospholipase C Gene at the Onset of the Stationary Phase. Journal of bacteriology 178:2749-2756.