Part:BBa_K389411:Design

vir promoter + sensitivity tuner + luciferase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2428
  Illegal NgoMIV site found at 3772
  Illegal NgoMIV site found at 3793
  Illegal AgeI site found at 1951
  Illegal AgeI site found at 2063
  Illegal AgeI site found at 2265
  Illegal AgeI site found at 3496
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3678
  

Design Notes

a modified sensitivity tuner BBa_I746370 to enhance the luciferase signal which interacts as a reporter for a vir promoter

Source

• luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
• terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
• sensitivity tuner part consists inter alia of
  • ogr activator from phage P2 (BBa_I746350)
  • PF promoter from phage P2 (BBa_I746360)
• virG was synthesized by Mr. Gene (BBa_K389002)
• vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389003)

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]