![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
B-carotene
Part:BBa_K343006:Design
Designed by: Christian Kurtzhals Group: iGEM10_SDU-Denmark (2010-10-14)
Expresses B-carotene monooxygenase on a constitutive promotor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 765
Illegal BamHI site found at 500
Illegal BamHI site found at 1757 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1361
Illegal BsaI site found at 1809
Design Notes
The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.
Source
The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.