Part:BBa_K343006:Design
Expresses B-carotene monooxygenase on a constitutive promotor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 765
Illegal BamHI site found at 500
Illegal BamHI site found at 1757 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1361
Illegal BsaI site found at 1809
Design Notes
1. Source and function
The gene was cloned from Drosophila melanogaster cDNA aquired from Drosophila Genomic Resource Center. The normal function of the gene is to create beta-caroten 15'15-monooxygenase as outlined above. The function of this gene has been characterized in the literature.
2. Modifications before assembly
Appart from addition of BioBrick prefix/suffix no changes were made to the DNA before inserting it into E. coli.
3. Choice of additional parts
We have chosen the J13002 promotr+rbs to avoid both having to ligate promotor and rbs seperately. The B0015 dual terminator because of good experiences from earlier teams.
4. Vector
The gene was inserted into a pSB1C3 backbone.
5. Safety considderations
We considered the gene, the strains of E. coli and plasmids used as safe.