Translational_Unit

Part:BBa_K329003

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-12)
Revision as of 16:19, 12 October 2010 by Registry (Talk | contribs)

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Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)

Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Strong RBS upstream (BBa_B0034). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see BBa_K329000)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 162
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 334
    Illegal NgoMIV site found at 702
    Illegal NgoMIV site found at 862
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None