Tag

Part:BBa_J70621

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-08)
Revision as of 15:06, 9 August 2010 by Jolynch (Talk | contribs)

RFC12 7x His Tag Tail Domain

This is a RFC12 compatible version of BBa_T2000. It is a 7x his tag tail domain meant for C-terminal fusions. His tags are meant to help purify a target protein.

Usage and Biology

Polyhistidine tags are a very common purification tag used to separate or identify a target protein.

Common tag information:

  • Matrix: Divalent Metal (Ni2+ is most common)
  • Yield/Purity: 20%-80% pure, but depending on the methodology can be higher
  • # Steps: One step
  • Fusion Location: C-terminus (N-terminus is BBa_J70609)
  • Affects Function?: Possible, but rare

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None