Part:BBa_J70621

RFC12 7x His Tag Tail Domain

This is a RFC12 compatible version of BBa_T2000. It is a 7x his tag tail domain meant for C-terminal fusions. .

Usage and Biology

Polyhistidine tags are a very common purification tag used to separate or identify a target protein.

Common tag information:

• Matrix: Divalent Metal (Ni²⁺ is most common)
• Yield/Purity: 20%-80% pure, but depending on the methodology can be higher
• # Steps: One step
• Fusion Location: C-terminus (N-terminus is BBa_J70609)
• Affects Function?: Possible, but rare

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]