DNA
pSC101 ts

Part:BBa_I50052

Designed by: Reshma Shetty   Group:   (2010-06-21)
Revision as of 18:25, 21 June 2010 by Rshetty (Talk | contribs) (→‎Usage and Biology)

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pSC101 temperature sensitive origin of replication

BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector.

Usage and Biology

This origin propagates at the permissive temperature of 30°C. At 37°C, the restrictive temperature, the repA protein becomes non-functional so that plasmids containing this origin can no longer propagate. It will take a few cell divisions for plasmid-free cells to arise and overtake the population.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/component/origin
Parameters
None