DNA
pSC101 ts

Part:BBa_I50052:Design

Designed by: Reshma Shetty   Group:   (2010-06-21)
Revision as of 18:23, 21 June 2010 by Rshetty (Talk | contribs) (→‎Source)

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pSC101 temperature sensitive origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See design notes for Part:BBa_I50042.


Source

This part is derived from BBa_I50042. It contains a point mutation that changes Alanine to Valine in RepA protein.

Generated using the following mutagenesis primers and the Stratagene QuickChange protocol:

Mutation-forward: 5' cgttcacttataaccaatacgttcagatgatgaacatcagtag 3'

Mutation-reverse: 5' ctactgatgttcatcatctgaacgtattggttataagtgaacg 3'

References