Coding

Part:BBa_K294208:Design

Designed by: Reshma Shetty   Group: iGEM09_Example   (2010-05-22)
Revision as of 23:00, 22 May 2010 by Rshetty (Talk | contribs)

luxC gene, acyl-CoA reductase LuxC from Vibrio fischeri ES114


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a 7xhis tag to the N terminus. I changed the stop codon to TAATAA.


Source

I obtained this sequence from Genbank accession no Y00509.

References

AUTHORS Engebrecht,J. and Silverman,M.

TITLE Nucleotide sequence of the regulatory locus controlling expression 

           of bacterial genes for bioluminescence

JOURNAL Nucleic Acids Res. 15 (24), 10455-10467 (1987)

PUBMED 3697093