Coding

Part:BBa_K5051001:Design

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-01)
Revision as of 12:37, 2 October 2024 by Thegodofsheep (Talk | contribs) (→‎References)


PPTase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 924
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 981
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for Saccharomyces cerevisiae


Source

Aureobasidium pullulans EXF-150 genomic sequence

References

1. Sunbul, M., Zhang, K., & Yin, J. (2009). Chapter 10 Using Phosphopantetheinyl Transferases for Enzyme Posttranslational Activation, Site Specific Protein Labeling and Identification of Natural Product Biosynthetic Gene Clusters from Bacterial Genomes. Methods in Enzymology on CD-ROM/Methods in Enzymology, 255–275. https://doi.org/10.1016/s0076-6879(09)04810-1

2. Venkitasubramanian, P., Daniels, L., & Rosazza, J. P. (2006). Reduction of carboxylic acids by nocardia aldehyde oxidoreductase requires a phosphopantetheinylated enzyme. Journal of Biological Chemistry, 282(1), 478–485. https://doi.org/10.1074/jbc.m607980200

3. Xue, S., Chi, Z., Zhang, Y., Li, Y., Liu, G., Jiang, H., Hu, Z., & Chi, Z. (2018). Fatty acids from oleaginous yeasts and yeast-like fungi and their potential applications. Critical Reviews in Biotechnology, 38(7), 1049–1060. https://doi.org/10.1080/07388551.2018.1428167