Part:BBa_K5051001:Design

PPTase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 924
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 981
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimised for Saccharomyces cerevisiae

Source

Aureobasidium pullulans EXF-150 genomic sequence

References

1. Lin, Z., Akin, H., Rao, R., Hie, B., Zhu, Z., Lu, W., Smetanin, N., Verkuil, R., Kabeli, O., Shmueli, Y., Costa, A. D. S., Fazel-Zarandi, M., Sercu, T., Candido, S., & Rives, A. (2023). Evolutionary-scale prediction of atomic-level protein structure with a language model. Science, 379(6637), 1123–1130. https://doi.org/10.1126/science.ade2574

2. Sunbul, M., Zhang, K., & Yin, J. (2009). Chapter 10 Using Phosphopantetheinyl Transferases for Enzyme Posttranslational Activation, Site Specific Protein Labeling and Identification of Natural Product Biosynthetic Gene Clusters from Bacterial Genomes. Methods in Enzymology on CD-ROM/Methods in Enzymology, 255–275. https://doi.org/10.1016/s0076-6879(09)04810-1

3. Venkitasubramanian, P., Daniels, L., & Rosazza, J. P. (2006). Reduction of carboxylic acids by nocardia aldehyde oxidoreductase requires a phosphopantetheinylated enzyme. Journal of Biological Chemistry, 282(1), 478–485. https://doi.org/10.1074/jbc.m607980200

4. Xue, S., Chi, Z., Zhang, Y., Li, Y., Liu, G., Jiang, H., Hu, Z., & Chi, Z. (2018). Fatty acids from oleaginous yeasts and yeast-like fungi and their potential applications. Critical Reviews in Biotechnology, 38(7), 1049–1060. https://doi.org/10.1080/07388551.2018.1428167