Composite

Part:BBa_K5198107

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)
Revision as of 11:08, 2 October 2024 by Rk320 (Talk | contribs)


nSEAP-NanoLuc
nSEAP-NanoLuc is the NanoLuciferase (BBa_K1734004 ) with native SEAP tag (BBa_K5198010 ). Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a continuous quantitative reporter for gene expression by repeatedly assessing the activity of certain promoter/enhancer elements in many cell samples. SEAP is contained in exosomes and is secreted into the extracellular space. nSEAP tag sequence causes extacellular secretion of proteins. NanoLuciferase is a highly-stable enzyme that functions as a luminescent reporter. Its luminesecnt reaction is ATP-independent and very sensitive. The small size and extreme brightness of NanoLuc makes it an ideal fusion partner. When attached to the nSEAP tag, this nSEAP-NanoLuc signal peptide can be used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 1540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 901
    Illegal BsaI.rc site found at 1390


[edit]
Categories
Parameters
None