Part:BBa_K5198010

native SEAP tag

Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP).

SEAP is a truncated form of human placental alkaline phosphate with a GPI anchor deletion, which allows the protein to be secreted into the cell culture supernatant. The N-terminal fragment of the signal peptide from SEAP is contained in exosomes and secreted into extracellular fluid. When added to the N-terminus, the native SEAP tag sequence causes extracellular secretion of proteins.

For our project's Solubility Study, we aim to investigate the secretion efficiency of the native SEAP tag in comparison with various other signal peptide tags when attached to different cargo proteins, including sfGFP, alkaline phosphatase, nanoluciferase, and beta-galactosidase. The goal is to identify signal peptides for most efficient secretion of soluble antigens to improve the ATLAS system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]