Coding
Adh

Part:BBa_K5291049:Design

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)
Revision as of 03:18, 2 October 2024 by Kortybones (Talk | contribs) (→‎Design Notes)


AdhA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB2-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis， pAB1-CYPY96F-VHB and pAB1-AlkB2-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AdhA is a significant component in degradation module.

Source

Acinetobacter venetianus RAG-1

References