Part:BBa_K5291049:Design
AdhA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 769
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In degradation module we constructed 4 main plasmids. Two PE degrading enzymes, CYPY96F-VHB and AlkB2-Rd45-AdhA, are designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer. In order to express above two enzymes in our chassis, pAB1-CYPY96F-VHB and pAB1-AlkB2-Rd45-VHB were constructed. pAB1-pS-PEBP-PEase was constructed to depolymerize PE microplastics. In addition, pAB1-PEBP-GFP was constructed to verify the adsorption capacity of PEBP to PE by fluorescence microscopy. AdhA is a significant component in degradation module.
Source
Acinetobacter venetianus RAG-1
References
Chen, S., et al. (2024). "An NAD(+)-dependent group Ⅲ alcohol dehydrogenase involved in long-chain alkane degradation in Acinetobacter venetianus RAG-1." Enzyme Microb Technol 172: 110343.