Intermediate

Part:BBa_K218004:Design

Designed by: Jeremy Kubik   Group: iGEM09_Calgary   (2009-10-19)
Revision as of 02:59, 22 October 2009 by Jfkubik (Talk | contribs) (→‎Design Notes)

Terminator followed by constitutive TetR promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The strong terminator is followed by a constitutive promoter so that the transcription of the gene upstream of the terminator is adequately insulated to not influence transcription of the gene downstream of the TetR promoter. This intermediate is constructed to allow for the construction of Part:BBa_K218006.

Source

source

References