Intermediate

Part:BBa_K218004:Design

Designed by: Jeremy Kubik   Group: iGEM09_Calgary   (2009-10-19)

Terminator followed by constitutive TetR promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The strong terminator is followed by a constitutive promoter so that the transcription of the gene upstream of the terminator is adequately insulated to not influence transcription of the gene downstream of the TetR promoter. This intermediate is constructed to allow for the construction of Part:BBa_K218006.

Source

B0015 - Registry of Standard Biological Parts

R0040 - Registry of Standard Biological Parts

References