Part:BBa_K292003

Kanamycin resistance cassette and a double terminator

This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional.

This part is completed by BBa_K292004] to finish the transcription before the resistance cassette.

Usage and Biology

This BioBrick gives kanamycin resistance.

The cassette also can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage.

In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin.

Functional Parameters

Theres is no special functional parameters.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

biology	T7TE-LuxIA
direction	Bidirectional
forward_efficiency	0.604[CC]/0.96[JK]
o_h	-NA-
protein	kanamycin resistance protein
rbs	-NA-
reversed_version	2145
reverse_efficiency	0.86[JK]
tag	None