Part:BBa_K292004

Double terminator + Kanamycin resistance cassette + Double ter

This part contains a double terminator and a functional kanamycin resistance cassette (promoter, resistance against kanamycin and a double terminator, BBa_B0014 + BBa_P1003 + BBa_B0014.

Usage and Biology

It is reliable to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage.

In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin.

We can use this part as a reporter, or as a simple resistance cassette.

The double terminator allows the RNA translation arrest before the resistance cassette, so this is already created to insert a coding gene with its RBS before the resistance cassette.

Functional Parameters

The resistance cassette has got a terminator so it is functionable.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

biology	T7TE-LuxIA
direction	Bidirectional
forward_efficiency	0.604[CC]/0.96[JK]
o_h	-NA-
protein	kanamycin resistance protein
rbs	-NA-
reversed_version	2145
reverse_efficiency	0.86[JK]
tag	None