Intermediate

Part:BBa_K292002

Designed by: David Charoy   Group: iGEM09_SupBiotech-Paris   (2009-10-19)
Revision as of 23:19, 21 October 2009 by DavidC (Talk | contribs)

pLac (LacI regulated) + Strong RBS


This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli.

Usage in and Biology

This promoter is really useful to control a gene expression by inactivating it with LacI repressor. When LacI is absent the gene should be expressed, and in the presence of LacI, pLac is inhibited. So this part is really suitable for gene expression control by inactivation of pLac with LacI.

We can use this part to control a failed feedback mechanism which induces the Lambda phage lytic cycle.

In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).

This part already contains a strong RBS to begin directly a gene transcription.

Functional Parameters

This BioBrick must be use with the LacI from E.coli BBa_C0012 [1].


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 110
    Illegal NheI site found at 133
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


biology-NA-
controlIPTG, lacI
directionForward
efficiency0.6
negative_regulators1
o_h-NA-
o_l-NA-
positive_regulators-NA-
rbs-NA-
[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//classic/regulatory/uncategorized
Parameters
biology
controlIPTG, lacI
directionForward
efficiency0.6
negative_regulators1
o_h
o_l
positive_regulators
rbs