Part:BBa_K292001

Double terminator + Constitutive promoter + Strong RBS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 110
  Illegal NheI site found at 133
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

This part contains a double terminator and a constitutive promoter family member (prokaryote)and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It comes from BBa_J23100.

Usage and Biology

It is a simple promoter in the goals to begin gene transcription with terminator before it. The BioBrick can be usefull if we want to add a coding gene in front of the BioBrick and start a new transcription.

Sources

BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16).
BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04).
Isolated from a library of promoters.