Composite
Part:BBa_K5324017:Design
Designed by: Sebastian Nolte Group: iGEM24_Alma (2024-09-26)
TEVp Secretion Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 600
Illegal NgoMIV site found at 906
Illegal AgeI site found at 261 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Influenced by 2019 Ionis Paris team with their use of the TEVp part BBa_K3102012.
Source
TEVp originates from tobacco etch virus
References
1 - Golda, M. et al. (2024) ‘Specificity of the mutant tobacco etch virus protease’, Proteins: Structure, Function, and Bioinformatics, 92(9), pp. 1085–1096. doi:10.1002/prot.26693