Coding

Part:BBa_K3102012

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)

TEV Protease

TEV protease is the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than the sequence of factor Xa, thrombin, or enterokinase. TEV protease is a very useful reagent for cleaving fusion proteins.

Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 492
    Illegal NgoMIV site found at 798
    Illegal AgeI site found at 153
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None