Part:BBa_K5415004:Design

HIF1 system for yeast humanisation.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3244
  Illegal BglII site found at 3452
  Illegal BamHI site found at 3355
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 929
  Illegal NgoMIV site found at 3383
  Illegal AgeI site found at 2972
  Illegal AgeI site found at 4459
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcgctctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattcttcattgttgtagctgttgctctgatctcccagcatggacagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatgaggctgctggccctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggtgagactagggtaggcagcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaactgggaagtacgagtcttagcagtagcctgggtagccacctgctgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggtggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttacaggaggggctaggccactattcctgaaattctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatccgcattgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggttgtcacaggctgaaccacctgggaatgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgccagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgtggcctctggattgtgttggagagtgtaggccgtggttcttggctagagttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggttctcatccagagccattcttggttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatgacaaaattctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgttgtgtcgggagatgaactctgttggttgacaaacattactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaacttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccaggccctgatgtagcctgtgcagtggaccaccacgaagtgaggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagagctactgccacacctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggatacgccctgtcagggcattttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattcagactgtggctggttcaaaacaggagtcacggagtcagacacatacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctccaagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttgccagttccccgcaaggacttcatgtgagaaactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatcttgttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgttcccttctccatcatcatcaaaatccagccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccagaggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggcagtagtcgccgccatcgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacacaacatatctaatcttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaaatggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaaagaaaagtctcgtgatgcagccagatctcggcgaagtaaagaatctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgaggcttaccatcagctatttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggttttgttatggttctcacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtgtttgattttactcatccatgtgaccatgaggaaatgagagaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcgaagtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgcaacatggaaggtattgcactgcacaggccacattcacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctcacccatcaaatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatgggatatgagccagaagaacttttaggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaaggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtggatatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactctcaaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttcaacaaacagaatgtgtccttaaaccggttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgctttaactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtaccattatataatgatgtaatgctcccctcacccaacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccacttcgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaatccagagtcactggaactttcttttaccatgccccagattcaggatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgtggatagtgatatggtcaatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatgttagctccctatatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagtcctcaaagcacagttacagtattccagcagactcaaatacaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtgacaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataaagaaactacaagtgccacatcatcaccatatagagatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgttatctgtcgctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggttcactttttcaagcagtaggaattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaatctagtgaacagaatggaatggagcaaaagacaattattttaataccctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggattaccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaagaattactcagagctttggatcaagttaacCACCACCACCATCACCACtgagcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat

Source

We utilised two key iGEM BioBricks: HIF-1 beta and HIF-1 alpha