Part:BBa_K5415004:Design
HIF1 system for yeast humanisation.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3244
Illegal BglII site found at 3452
Illegal BamHI site found at 3355 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 3383
Illegal AgeI site found at 2972
Illegal AgeI site found at 4459 - 1000COMPATIBLE WITH RFC[1000]
ADH1 terminator:
ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgt
tttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgc
HIF-1beta:
ctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattcttcattgttgtagctgttgctctgatctcccagcatgga
cagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatgaggctgctggcc
ctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggt
gagactagggtaggcagcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaa
ctgggaagtacgagtcttagcagtagcctgggtagccacctgctgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggt
ggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttacaggaggggctaggccactattcctgaaatt
ctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatccgc
attgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggt
tgtcacaggctgaaccacctgggaatgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgc
cagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgtggcctctggattgtgttggagagtgtaggccgtggttcttggctaga
gttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggttctcatccagagccattcttg
gttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatg
acaaaattctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgtt
gtgtcgggagatgaactctgttggttgacaaacattactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaa
cttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccaggccctgatgtagcctgtgcagtggaccaccacgaagtg
aggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagagctactgccaca
cctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggat
acgccctgtcagggcattttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattc
agactgtggctggttcaaaacaggagtcacggagtcagacacatacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctc
caagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttgccagttccccgcaaggacttcatgtgaga
aactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatctt
gttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgtt
cccttctccatcatcatcaaaatccagccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccaga
ggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggcagtagtcgccgccat
GAL1,10 promoter:
cgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacac
aacatatctaatcttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaa
ctgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtc
gcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcag
taacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatc
agcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattactt
cttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaa
HIF-1alpha
atggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaaagaaaagtctcgtgatgcagccagatctcggcgaagtaaagaa
tctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgaggcttaccatcagctat
ttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggtttt
gttatggttctcacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtg
tttgattttactcatccatgtgaccatgaggaaatgagagaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcga
agtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgcaacatggaaggtattgcactgcacaggccacatt
cacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctcacccatca
aatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatggga
tatgagccagaagaacttttaggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaa
ggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtggatatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactct
caaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttcaacaaacagaatgtgtccttaaaccg
gttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgct
ttaactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtacca
ttatataatgatgtaatgctcccctcacccaacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccactt
cgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaatccagagtcactggaactttcttttaccatgccccagattcag
gatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgtggatagtgatatggtc
aatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatg
ttagctccctatatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagt
cctcaaagcacagttacagtattccagcagactcaaatacaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtg
acaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataaagaaactacaagtgccacatcatcaccatataga
gatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgttatctgtc
gctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggt
tcactttttcaagcagtaggaattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaa
tctagtgaacagaatggaatggagcaaaagacaattattttaataccctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggatta
ccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaagaattactcagagctttggatcaagtt
aacCACCACCACCATCACCACtga
ADH2 terminator
gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaa
taaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtaga
catggtagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat