Reporter

Part:BBa_K292004:Design

Designed by: David Charoy   Group: iGEM09_SupBiotech-Paris   (2009-10-19)
Revision as of 21:20, 21 October 2009 by DavidC (Talk | contribs) (→‎Source)

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Double terminator + Kanamycin resistance cassette + Double ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid pSB1AK3. But it's worked with a pSB1A1.

BBa_P1003 is an kanamycin antibiotic resistance cassette. Several silent mutations have been introduced to eliminate useful restriction sites and rare codons. However, we inadvertently introduced a new BsmBI recognition site in BBa_P1003 (kanamycin antibiotic resistance marker) during the course of codon shuffling.

Source

BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23)

References