Composite

Part:BBa_K5269020

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)
Revision as of 03:43, 29 September 2024 by Nakaiyuichiro (Talk | contribs)


Golden Gate Assembly Site(tac promoter, BsmB I insert, L3S2P21)

This sequence contains a tac promoter(BBa_K5269033), a BsmB I insert(BBa_K5269032), and a L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the Surface displayed protein (BBa_K5269021-BBa_K5269024).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BamHI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None