Composite

Part:BBa_K5269020

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


Golden Gate Assembly Site(tac promoter, BsmB I insert, L3S2P21)

This sequence contains a tac promoter, a BsmB I insert(BBa_K5269032), and a L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the Surface displayed protein (BBa_K5269021-BBa_K5269024).

Here we show an image of the Golden Gate Assembly Site amplified by PCR and detected by electrophoresis. See the experiments page (https://2024.igem.wiki/tokyotech/experiments/) for details.

SDS-PAGE Image

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BamHI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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