Composite

Part:BBa_K5269020

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)
Revision as of 13:44, 28 September 2024 by Registry (Talk | contribs)

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Golden Gate Assembly Site(tac promoter, BsmB I insert, L3S2P21)

This sequence contains a tac promoter, a BsmB I cleavage site, and an L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I cleavage sites were used for Golden Gate Assembly of the surface-presenting proteins (BBa_K5269021-BBa_K5269024).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BamHI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None