Coding

Part:BBa_K5477022

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 18:18, 26 September 2024 by Registry (Talk | contribs)

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POR - Cytochrome P450 oxidoreductase codon optimized for Saccharomyces cerevisiae

Cytochrome P450 oxidoreductase (POR) is an essential enzyme that provides the necessary electrons to support the function of cytochrome P450 enzymes in their metabolic activities. POR is a flavoprotein, containing both FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide) cofactors, which are critical for transferring electrons from NADPH to cytochrome P450 enzymes. This electron transfer is necessary for the P450 enzymes to carry out their role in phase I metabolism, specifically the oxidation of a wide variety of substrates, including drugs, toxins, and endogenous compounds. POR is localized in the endoplasmic reticulum of cells, where it interacts with multiple cytochrome P450 enzymes, including CYP3A4, CYP1A1, and others. By delivering the electrons required for these enzymes to function, POR facilitates the catalytic cycle of cytochrome P450s, which involves the incorporation of molecular oxygen into substrates, often resulting in hydroxylated metabolites. These metabolites are usually more hydrophilic and can be further processed by phase II enzymes, such as UGTs (UDP-glucuronosyltransferases), making them easier to excrete from the body.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
    Illegal NgoMIV site found at 699
    Illegal NgoMIV site found at 818
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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