Coding

Part:BBa_K5477022

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)


POR - Cytochrome P450 oxidoreductase codon optimized for Saccharomyces cerevisiae

Cytochrome P450 oxidoreductase (POR) provides the necessary electrons to support the function of cytochrome P450 enzymes in their metabolic activities(1) (2). POR is a flavoprotein, containing both FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide) cofactors, which transfers electrons from NADPH to cytochrome P450 enzymes (1) (2). This electron transfer is necessary for the P450 enzymes in phase I metabolism, specifically the oxidation of a wide variety of substrates, including drugs, toxins, and endogenous compounds (1) (2).

This part was combined with CYP3A4 or CYP1A1. The following composites and devices, where this part was used, are listed here: BBa_K5477037, BBa_K5477038, BBa_K5477039 and BBa_K5477047


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 296
    Illegal PstI site found at 493
    Illegal PstI site found at 877
    Illegal PstI site found at 1317
    Illegal PstI site found at 1377
    Illegal NgoMIV site found at 699
    Illegal NgoMIV site found at 818
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Pandey AV, Flück CE. NADPH P450 oxidoreductase: structure, function, and pathology of diseases. Pharmacol Ther. 2013;138(2):229-254. doi:10.1016/j.pharmthera.2013.01.010

2. Pandey AV, Sproll P. Pharmacogenomics of human P450 oxidoreductase. Front Pharmacol. 2014 May 9;5:103. doi: 10.3389/fphar.2014.00103. PMID: 24847272; PMCID: PMC4023047.

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