Part:BBa_K5291042
CYPY96F
CYPY96F is a protein-engineered variant of the cytochrome P450cam enzyme, designed to enhance its ability to catalyze the oxidation of alkanes. The Y96F mutation introduces phenylalanine at position 96, increasing the substrate-binding pocket's hydrophobicity. This modification is crucial for improving the enzyme's affinity for hydrophobic substrates like alkanes and reducing the enzyme's susceptibility to uncoupling, where water molecules interfere with the catalytic cycle. The CYPY96F variant shows significantly increased activity and product formation rates for alkane oxidation compared to the wild-type enzyme. Researchers and bioengineers can incorporate CYPY96F into projects that require selective oxidation of alkanes, such as in the synthesis of bioremediation, biofuels, or other specialty chemicals.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 312
Illegal BamHI site found at 392
Illegal BamHI site found at 1221 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 109
Illegal NgoMIV site found at 277 - 1000COMPATIBLE WITH RFC[1000]
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