Coding
CYP
Part:BBa_K5291042
Designed by: Wendan Zheng Group: iGEM24_BNUZH-China (2024-09-26)
CYPY96F
CYPY96F: P450cam mutant with higher alkane oxidation activity.
Usage and Biology
In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.
Fig.1 The map of plasmid pAB1-CYPY96F-VHb.
Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.
Fig.2 The SDS-PAGE result of CYPY96F.
Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 312
Illegal BamHI site found at 392
Illegal BamHI site found at 1221 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 109
Illegal NgoMIV site found at 277 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |