Part:BBa_K5349005:Design

UL23-sfGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1611
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 879
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service

Source

• UL23 : Refer to K5349000 https://parts.igem.org/Part:BBa_K5349000:Design
• sfGFP : Refer to K2553008 https://parts.igem.org/Part:BBa_K2553008:Design