Coding

Part:BBa_K5349000:Design

Designed by: Tomoharu Sakata   Group: iGEM24_BotchanLab-Tokyo   (2024-09-26)


Kill switch, HSV-TK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon-optimized for expression in E. coli, based on the DNA sequence obtained from Nactional Ceneter of Biotechnology Information (NCBI). However, some nucleotides were additionally replaced to reduce synthesis difficulty below a certain threshold when using Twist Bioscience's DNA synthesis service.


Source

This thymidine kinase is encoded by a gene derived from the herpes simplex virus

References