Part:BBa_K5115026

csoS2

Introduction

CsoS2 is an essential protein involved in the assembly and formation of α-carboxysomes, which are bacterial microcompartments designed to encapsulate enzymes such as ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) for carbon fixation. The carboxysome shell, composed of various proteins, serves as a selectively permeable barrier, facilitating the flux of specific metabolites while protecting enzymes from external inhibitors like oxygen. CsoS2 plays a crucial role in mediating the self-assembly of the shell and is functionally analogous to linking proteins found in β-carboxysomes, like CcmM. In the chemoautotrophic bacterium Halothiobacillus neapolitanus, CsoS2 is present in two isoforms (CsoS2A and CsoS2B), which together drive the formation of ordered, virus-like protein shells in E. coli.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 133
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 291
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 799
  Illegal AgeI site found at 1750
  Illegal AgeI site found at 2431
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 191