Coding
Part:BBa_K5115026
Designed by: Liyue Chen Group: iGEM24_Fudan (2024-09-18)
csoS2
Introduction
The csoS2 is a subunit of the csoS operon BBa_K5115034.
Usage and Biology
The csoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, csoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, csoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.
Characterization
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 133
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 291
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 799
Illegal AgeI site found at 1750
Illegal AgeI site found at 2431 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 191
References
[edit]
Categories
Parameters
None |