Part:BBa_K5205001

Non, signal-like sequence

The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) (BBa_K5205002) to form the Non-OmpA system (BBa_K5205003). This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).

References

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0