Coding

Part:BBa_K5374010:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-12)
Revision as of 09:10, 19 September 2024 by Francisgrace (Talk | contribs) (→‎Source)


SPARC/OD-EGFP. A collagen-binding domain involved in tissue repair and bone mineralization, fused wi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 484
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 484
    Illegal NheI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 484
    Illegal BglII site found at 897
    Illegal BamHI site found at 288
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 484
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 484
    Illegal NgoMIV site found at 859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1565


Design Notes

None


Source

The SPARC domain is derived from the human SPARC gene. EGFP is sourced from BBa_K1911005, a widely used fluorescence marker.

References