Coding
Part:BBa_K5374010:Design
Designed by: Langning Xu Group: iGEM24_TestDaily-I-Beijing (2024-09-12)
SPARC/OD-EGFP. A collagen-binding domain involved in tissue repair and bone mineralization, fused wi
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 484
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 484
Illegal NheI site found at 922 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 484
Illegal BglII site found at 897
Illegal BamHI site found at 288 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 484
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 484
Illegal NgoMIV site found at 859 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1565
Design Notes
The SPARC/OD sequence was optimized for fusion with EGFP to maintain collagen-binding activity without disrupting EGFP fluorescence, providing insight into the impact of SPARC as a release tag for cell factors.
Source
The SPARC domain is derived from the human SPARC gene. EGFP is sourced from BBa_K1911005, a widely used fluorescence marker.