Regulatory
TrpR

Part:BBa_K191007

Designed by: Le Thanh Tu NGUYEN   Group: iGEM09_EPF-Lausanne   (2009-10-16)
Revision as of 13:49, 21 October 2009 by Nltt1987 (Talk | contribs)

Trp promoter

Promoter of Tryptophan operon ( Tryptophan operon is an operon that codes for the components of tryptophan's synthesis)

Usage and Biology

Presence of Tryptophan inhibits the expression of genes downstream of the regulator. As the hybrid protein LovTAP ( BBa_K191006 ) contains the corresponding regulatory domain, LovTAP can also bind to the regulator and and interferes with downstream gene's expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/negative
Parameters
input_sTrp, LovTAP
negative_regulators