Part:BBa_K191006

LOVTAP

Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki

Improvements

Part:BBa_K838000. An implementation of the LovTAP protein for mammalian cells.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 141
  Illegal PstI site found at 398
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 141
  Illegal PstI site found at 398
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 141
  Illegal PstI site found at 398
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 141
  Illegal PstI site found at 398
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 535