Composite

Part:BBa_K243024

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-18)
Revision as of 13:35, 21 October 2009 by Timo (Talk | contribs)

Strep-FluA-Middle Linker-Fok_a

This combination of parts use the benefits of an Strep-tag for purification. It is also linked with a FluroescineA tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee the independent function of FluA tag and the protein domain Fok_a.The fluoresceinA tag allows the measurement by [quenching] and the coupling to an flurescein linked oligo.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1075


[edit]
Categories
Parameters
n/apMA Strep-FluA-Middle Linker-Fok_a