Protein_Domain

Part:BBa_K157004

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)

Fluoresceine-A-binding


[http://2013.igem.org/Team:TU-Munich Team TU Munich 2013] provides a higher engineered variant of this anticalin, which is also compatible to RFC[10] and RFC[25] (see: BBa_K1159003) and has a higher affinity to fluorescein.
(--TU Munich 19:30, 27 September 2013 (UTC))


Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from Pieris brassicae, originally designed by Arne Skerra [see Ref. 1,2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
//function/reporter
//proteindomain/binding
//proteindomain/internal
Parameters
None