Part:BBa_K5317002
miRFP670
Usage and Biology
The monomeric near-infrared (NIR) fluorescent protein miRFP670 was developed 2016 from Shcherbakova and colleagues. The exciation maxima of miRFP670 is at 642 nm and the emission maxima at 670 nm. miRFP670 was genetically engineered from bacterial phytochrome photoreceptors (BphP) to generate reporters using the opotical window with low autofluorescence, decreased light scattering and minimal absorption of biological substances such as haemoglobin, water and melanin. Of the BphP-based fluorophores, miRFP is particularly suitable for investigations in mammalian cell lines due to a fluorescence quantum yield of 14 % (Shcherbakova et al. 2016).
miRFP670 was employed in our mammalian cell-based antibiotic detection sensor downstream of the ATF2-3xCre2xAP1 promoter visualizing the antiobiotic presence-dependent phosphorylation of ATF2.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 763
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 763
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 419
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 763
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 763
Illegal NgoMIV site found at 82
Illegal NgoMIV site found at 370
Illegal NgoMIV site found at 405 - 1000COMPATIBLE WITH RFC[1000]
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