Part:BBa_K5317002

miRFP670

Usage and Biology

The monomeric near-infrared (NIR) fluorescent protein miRFP670 was developed by Shcherbakova and colleagues in 2016. The excitation maxima of miRFP670 is at 642 nm and the emission maxima at 670 nm. miRFP670 was genetically engineered from bacterial phytochrome photoreceptors (BphP) to generate reporters using the optical window with low autofluorescence, decreased light scattering, and minimal absorption of biological substances such as hemoglobin, water, and melanin. Of the BphP-based fluorophores, miRFP is particularly suitable for investigations in mammalian cell lines due to a fluorescence quantum yield of 14 % and maintaining brightness in the physiological pH range from 5 to 8 (Shcherbakova et al., 2016).

miRFP670 was employed in our mammalian cell-based antibiotic detection sensor downstream of the ATF2-3xCre3xAP1 promoter (K5317017) visualizing the antibiotic presence-dependent phosphorylation of ATF2 (K5317016).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 763
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 763
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 419
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 763
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 763
  Illegal NgoMIV site found at 82
  Illegal NgoMIV site found at 370
  Illegal NgoMIV site found at 405
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Shcherbakova, D. M., Baloban, M., Emelyanov, A. V., Brenowitz, M., Guo, P., & Verkhusha, V. V. (2016). Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Nature communications, 7, 12405. https://doi.org/10.1038/ncomms12405