Plasmid

Part:BBa_K5384007

Designed by: Si Cheng   Group: iGEM24_HBUT-Wuhan   (2024-08-24)
Revision as of 07:39, 24 August 2024 by Registry (Talk | contribs)

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pPIC9K-His-DDDDK-Vg

VGLYCIN gene and DDDDK site were linked to plasmid pPIC9K after inserting VGLYCIN coding polypeptide gene from Pichia pastoris plasmid. We designed the new plasmid in the experiment, because we need to transform Pichia pastoris to express our target protein VGLYCIN, the plasmid PIC9K-3His-DDDK-vg, which we constructed and used, has a replication origin of the Pichia pastoris plasmid, an AOX1 promotor promoter, an α-factor secretion signal replication signal, and a plasmid AOX1 terminator replication terminator, it is convenient to insert and express the target gene and screen out the recombinant. The Ori site of the Pichia pastoris plasmid was derived from the Pichia pastoris plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 142
    Illegal XhoI site found at 1342
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1121
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid
//plasmid/expression
Parameters
biology
functionIt can ecode a peptide,which has excellent hypoglycemic effect on mice and can repair damaged pancreas.Besides,it from soybean which means untoxic..
protein excellent hypoglycemic effect on mice and can repair damaged pancreas.