Part:BBa_K5384007

pPIC9K-His-DDDDK-Vg

Vglycin gene and DDDDK were inserted to the plasmid pPIC9K ang then transformed into Pichia pastoris. We designed the new plasmid in the experiment to massively produce Vg. The plasmid pPIC9K-His-DDDDK-Vg, which we constructed and used, has an AOX 1 promoter, an α-factor secretion signal, and a plasmid AOX 1 terminator, it is convenient to insert and express the target gene and purify the recombinant.

Figure 1 Visualization of the pPIC9K-His-DDDDK-Vg for overexpression

Usage and Biology

This composite part contains three His-tag (BBa_K5384006), the Vglycin gene (BBa_K5384001), the Enterokinase recognition site (BBa_K5384002), the Asp-pro acid-sensitive site (BBa_K5384003), and the AOX 1 promoter (BBa_K5384004), and α-secretion signal peptide (BBa_K5384005).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1121
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1121
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 142
  Illegal XhoI site found at 1342
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1121
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1121
  
• 1000
  COMPATIBLE WITH RFC[1000]

Application

After inserting the Vglycin-encoding polypeptide gene into the plasmid, we transformed the plasmid into Pichia pastoris to produce Vg. The plasmid pPIC9K-His-DDDDK-Vg we constructed and used has AOX 1 promoter, α-factor secretion signal, and plasmid AOX 1 terminator, which facilitates insertion and expression of target genes and purification of recombinants.

References

[1] WANG Wenli, WANG Yunlong, LI Chenyang, et al. Preparation, Identification and Preliminary Application of His-tagged Monoclonal Antibody[J]. J Cell and Molecular Immunol,2008,24(4):399-400. DOI:10.3321/j.issn:1007-8738.2008.04.028.

[2] LI Shuying, ZHAO Zhonglin, NIE Ying, et al. Research Progress on Nattokinase[J]. China Agricultural Science and Technology Review,2013,15(4):139-143.] DOI:10.3969/j.issn.1008-0864.2013.04.21.

[3] Huang Zhili, Luo Lixin, Yang Rude, et al. Nattokinase[J]. Chemistry of Life,2000(2):82-83.] DOI:10.3969/j.issn.1000-1336.2000.02.012.

[4] Zhao Fuyong, Yan Han, Ren Guangxu, et al. Research Progress of Recombinant Nattokinase[J]. China Food and Nutrition,2019,25(7):41-45.] DOI:10.3969/j.issn.1006-9577.2019.07.008. Establishment of nattokinase detection system and a preliminary study on its transmembrane transport pathway[J]. Journal of Chengdu Medical College,2016,11(5):532-536,564. DOI:10.3969/j.issn.1674-2257.2016.05.003.

[5] Tang Xiaoyan. Screening and application evaluation of efficient transcriptional termination sequences in Pichia pastoris[D]. Guangdong:South China University of Technology,2019(in Chinese).